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KMID : 1101420180500020170
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Korean Journal of Clinical Laboratory Science
2018 Volume.50 No. 2 p.170 ~ p.176
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Antioxidative Effects of Cornis fructus Extract on Chromium Trioxide Toxicity
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Seo Young-Mi
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Abstract
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This study examined the antioxidative effects of Cornis fructus (CF) extract on cultured C6 glioma cells. For this purpose, cytotoxicity analysis of chromium trioxide (CrO3) was performed and the protective effects of the CF extract on CrO3?induced cytotoxicity was examined after the C6 glioma cells were cultured for 48 hours. The antioxidative effects, such as electron donating activity (EDA) and lactate dehydrogenase (LDH) activity were also analyzed. In this study, CrO3 decreased the cell viability in a dose dependent manner. The XTT50 value was determined to be 33 μM after the cells were treated for 48 hours at a concentrations of 20∼40 μM CrO3. The catalase (CAT) antioxidant increased significantly the cell viability that had been decreased by CrO3?induced cytotoxicity. Regarding the protective effect of the CF extract, the cell viability of the CF extract was increased significantly compared to that of CrO3 only. In addition, the CF extract showed antioxidative effects, such as EDA and an inhibitory effect on the LDH activity. These findings suggest that the cytotoxicity of CrO3 is correlated with oxidative stress, and the CF extract effectively prevented CrO3?induced cytotoxicity through the antioxidative effects. In conclusion, natural products, such as the CF extract may be a useful therapeutic agent for the prevention or treatment of toxicity induced by heavy metals via oxidative stress.
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KEYWORD
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Antioxidative effect, Cytotoxicity, Natural products, Oxidative stress
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